Coding
T7pol

Part:BBa_K145001:Design

Designed by: Jonas Demeulemeester and Benjamien Moeyaert   Group: iGEM08_KULeuven   (2008-07-08)

T7 RNA polymerase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 polymerase from BBa_I712022 with NLS site removed and TAATAA double stop signal added. This was done with PCR using the following primers: 

primer 1: ATGAATTCGC GGCCGCTTCT AGATGAACAC GATTAACATC GC
primer 2: CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG

Source

T7 polymerase from BBa_I712022.

References